The Human Casein Kinase 1 Delta (CSNK1D) ELISA Kit is specially designed for the precise and reliable measurement of CSNK1D levels in human serum, plasma, and cell culture supernatants. This high-quality kit offers exceptional sensitivity and specificity, ensuring accurate and reproducible results for a variety of research applications.CSNK1D is a critical enzyme that plays a key role in regulating various cellular processes, including cell cycle progression, gene expression, and signal transduction. Dysregulation of CSNK1D has been linked to several diseases, such as cancer, neurological disorders, and metabolic diseases, making it a valuable biomarker for studying these conditions and potential therapeutic targeting.
With its user-friendly protocol and quick assay time, the Human CSNK1D ELISA Kit is an indispensable tool for researchers seeking to investigate the function and significance of CSNK1D in health and disease. Trust in the accuracy and precision of this kit to advance your research efforts and contribute to the understanding of CSNK1D-related pathways.
Product Name:
Human Casein Kinase 1 Delta (CSNK1d) ELISA Kit
Product Code:
HUDL00701
Size:
96 Assays
Target:
CSNK1d
Synonyms:
HCKID, Tau-protein kinase CSNK1D, Casein kinase I isoform delta
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
83-100
91
EDTA plasma (n=5)
90-103
96
Heparin plasma (n=5)
89-102
95
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
