Human Carnitine Palmitoyltransferase 1A, Liver (CPT1A) ELISA Kit
The Human Carnitine Palmitoyltransferase 1A Liver (CPT1A) ELISA Kit is specifically designed for the precise measurement of CPT1A levels in human serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for a wide range of research applications.CPT1A is a key enzyme involved in fatty acid metabolism, particularly in the liver where it regulates the uptake and oxidation of fatty acids. Dysregulation of CPT1A has been linked to metabolic disorders such as fatty liver disease and obesity, making it a crucial biomarker for studying these conditions and developing potential therapeutic interventions.
By utilizing the Human CPT1A ELISA Kit, researchers can accurately assess CPT1A levels in biological samples, providing valuable insights into the role of this enzyme in metabolic health and disease. With its high performance and reliability, this kit is an essential tool for advancing research in the field of lipid metabolism and metabolic disorders.
Product Name:
Human Carnitine Palmitoyltransferase 1A, Liver (CPT1A) ELISA Kit
Product Code:
HUDL00661
Size:
96 Assays
Target:
CPT1A
Synonyms:
CPT1-L, Carnitine O-palmitoyltransferase I, liver isoform
Detection Method:
Sandwich
Range:
0.781-50ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
83-100
91
EDTA plasma (n=5)
90-103
96
Heparin plasma (n=5)
89-102
95
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
