Human Carcinoembryonic Antigen Related Cell Adhesion Molecule 1 (CEACAM1) ELISA Kit

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SKU:
HUDL00524
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P13688
Sensitivity:
0.57ng/mL
Range:
1.56-100ng/mL
ELISA Type:
Sandwich
Synonyms:
CD66a,BGP,BGP1,BGPI,CD66-A,Biliary Glycoprotein
Reactivity:
Human
Sample Type:
Serum, Plasma And Other Biological Fluids
Frequently bought together:

Description

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Human Carcinoembryonic Antigen Related Cell Adhesion Molecule 1 (CEACAM1) ELISA Kit

The Human CEACAM1 (Carcinoembryonic Antigen-Related Cell Adhesion Molecule 1) ELISA Kit is specifically designed for the accurate quantification of CEACAM1 levels in human serum, plasma, and cell culture supernatants. This kit boasts high sensitivity and specificity, ensuring precise and dependable results for a variety of research applications.CEACAM1 is a key protein involved in cell adhesion and immune response regulation. It is implicated in various diseases including cancer, inflammatory disorders, and autoimmune conditions, making it a valuable biomarker for studying these diseases and developing targeted treatments.

With the Human CEACAM1 ELISA Kit, researchers can confidently measure CEACAM1 levels in biological samples, helping to advance scientific understanding and potentially leading to the discovery of new diagnostic and therapeutic strategies.

Product Name:Human Carcinoembryonic Antigen Related Cell Adhesion Molecule 1 (CEACAM1) ELISA Kit
Product Code:HUDL00524
Size:96 Assays
Target:CEACAM1
Synonyms:CD66a, BGP, BGP1, BGPI, CD66-A, Biliary Glycoprotein
Detection Method:Sandwich
Range:1.56-100ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10191
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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