The Human Carbonyl Reductase 3 (CBR3) ELISA Kit is a reliable tool for the precise measurement of CBR3 levels in human samples including serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit provides accurate and reproducible results, making it ideal for a variety of research applications.CBR3 is an important enzyme involved in the metabolism of carbonyl compounds, playing a role in detoxification processes and drug metabolism. Dysregulation of CBR3 has been linked to various diseases such as cancer, diabetes, and neurodegenerative disorders, highlighting its significance as a potential biomarker for disease progression and drug development.
By utilizing the Human Carbonyl Reductase 3 ELISA Kit, researchers can gain valuable insights into the role of CBR3 in disease pathogenesis and potentially identify new therapeutic targets for intervention. This kit is a valuable tool for studying CBR3 function and its implications in human health.
Product Name:
Human Carbonyl Reductase 3 (CBR3) ELISA Kit
Product Code:
HUDL00447
Size:
96 Assays
Target:
CBR3
Synonyms:
SDR21C2, Short Chain Dehydrogenase/Reductase Family 21C,Member 2, NADPH-dependent carbonyl reductase 3
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
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