Human Carbamoyl Phosphate Synthase 1, Mitochondrial (CPS1) ELISA Kit
The Human Carbamoyl Phosphate Synthase 1 (Mitochondrial) CPS1 ELISA Kit is a cutting-edge tool designed for the precise measurement of CPS1 levels in human biological samples such as serum, plasma, and cell culture supernatants. With exceptional sensitivity and specificity, this kit delivers consistent and accurate results, making it a valuable asset for various research endeavors.CPS1 is an essential enzyme responsible for the synthesis of carbamoyl phosphate, a key component in the urea cycle. Dysregulation of CPS1 activity has been linked to various metabolic disorders and liver diseases, underscoring the importance of monitoring CPS1 levels in clinical and research settings.
Whether investigating metabolic pathways, studying hepatic function, or exploring potential therapeutic interventions, the Human Carbamoyl Phosphate Synthase 1 (Mitochondrial) CPS1 ELISA Kit is an indispensable tool for researchers seeking to deepen their understanding of metabolic processes and related disorders. Order yours today and unlock new insights into the intricate biochemical mechanisms governing human physiology.
Product Name:
Human Carbamoyl Phosphate Synthase 1, Mitochondrial (CPS1) ELISA Kit
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
