Human Capping Protein Muscle Z Line Beta (CAPZb) ELISA Kit
The Human Muscle Z-line Beta CapZB ELISA Kit offered by Assay Genie is a cutting-edge tool for measuring levels of the capping protein muscle Z-line beta (CapZB) in human samples. This kit provides researchers with a highly sensitive and specific method for quantifying CapZB in serum, plasma, and cell culture supernatants, ensuring accurate and reliable results for a variety of research purposes.CapZB is a key component of the muscle Z-line, playing a crucial role in maintaining muscle structure and function.
Dysregulation of CapZB has been associated with various muscle disorders and diseases, making it an important biomarker for studying and diagnosing these conditions. By utilizing the Human Muscle Z-line Beta CapZB ELISA Kit, researchers can gain valuable insights into muscle health and potentially develop new therapeutic strategies for muscle-related disorders.
Product Name:
Human Capping Protein Muscle Z Line Beta (CAPZb) ELISA Kit
Product Code:
HUDL00431
Size:
96 Assays
Target:
CAPZb
Synonyms:
CAPZ, CAPB, CAPPB, F-Actin-Capping Protein Subunit Beta
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
