Calsequestrin-1 is an isoform of calsequestrin. Calsequestrin is a calcium-binding protein of the sarcoplasmic reticulum. It helps hold calcium in the cisterna of the sarcoplasmic reticulum after a muscle contraction, even though the concentration of calcium in the sarcoplasmic reticulum is much higher than in the cytosol. Two forms of calsequestrin have been identified: Calsequestrin-2 and Calsequestrin-1. Calsequestrin-1 is found in fast skeletal muscle. The release of calsequestrin-bound calcium (through a calcium release channel) triggers muscle contraction. The active protein is not highly structured, more than 50% of it adopting a random coil conformation. When calcium binds there is a structural change whereby the alpha-helical content of the protein increases from 3 to 11%. Both forms of calsequestrin are phosphorylated by casein kinase 2, but the cardiac form is phosphorylated more rapidly and to a higher degree. Calsequestrin-1 is also secreted in the gut where it deprives bacteria of calcium ions.
Product Name:
Human Calsequestrin/CASQ1 Recombinant Protein (RPES4009)
Product Code:
RPES4009
Size:
20µg
Species:
Human
Expressed Host:
E.coli
Synonyms:
CASQ,PDIB1,VMCQA
Accession:
P31415
Sequence:
Gln35-Asp396
Fusion tag:
Endotoxin:
Please contact us for more information.
Protein Construction:
A DNA sequence encoding the mature form of human CASQ1 (P31415) (Gln35-Asp396) was expressed with a N-terminal Met.
Purity:
> 90 % as determined by reducing SDS-PAGE.
Mol Mass:
41.8 kDa
AP Mol Mass:
52 kDa
Formulation:
Lyophilized from sterile 50mM Tris, 10% glycerol, pH 7.5
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
