Human Calcium Channel, Voltage Dependent, N-Type, Alpha 1B Subunit (CACNa1B) ELISA Kit
The Human Calcium Channel Voltage-Dependent N Type Alpha-1B Subunit (CACNA1B) ELISA Kit is a precise and reliable tool for the quantitative detection of CACNA1B levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers high specificity and sensitivity, ensuring accurate and reproducible results for a variety of research applications.CACNA1B is an important component of calcium channels involved in controlling calcium influx into cells, playing a crucial role in neuronal function and synaptic transmission.
Dysregulation of CACNA1B has been implicated in various neurological disorders, making it a valuable biomarker for understanding the pathophysiology of these conditions and potentially developing targeted therapies.Overall, the Human CACNA1B ELISA Kit provides researchers with a valuable tool for studying the role of calcium channels in health and disease, offering insights into potential diagnostic and therapeutic strategies for neurological disorders.
Product Name:
Human Calcium Channel, Voltage Dependent, N-Type, Alpha 1B Subunit (CACNa1B) ELISA Kit
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
83-100
91
EDTA plasma (n=5)
90-98
94
Heparin plasma (n=5)
89-102
95
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
