Human Calcium Channel, Voltage Dependent, L-Type, Alpha 1D Subunit (CACNa1D) ELISA Kit
The Human Calcium Channel Voltage Dependent L-Type Alpha 1D Subunit (CACNA1D) ELISA Kit is specifically designed for the quantitative detection of CACNA1D levels in human serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reliable results for a variety of research applications.CACNA1D is a key protein involved in calcium channel regulation, influencing processes such as muscle contraction, neurotransmitter release, and gene expression. Dysregulation of CACNA1D has been implicated in various diseases including cardiovascular disorders, neurological conditions, and certain types of cancer.
As such, accurate measurement of CACNA1D levels can provide valuable insights for understanding disease mechanisms and developing targeted therapies.By utilizing the Human Calcium Channel Voltage Dependent L-Type Alpha 1D Subunit (CACNA1D) ELISA Kit, researchers can confidently analyze CACNA1D expression levels in biological samples, advancing their investigations into the role of calcium channels in health and disease.
Product Name:
Human Calcium Channel, Voltage Dependent, L-Type, Alpha 1D Subunit (CACNa1D) ELISA Kit
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
83-100
91
EDTA plasma (n=5)
90-98
94
Heparin plasma (n=5)
89-102
95
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
