Human Calcium/Calmodulin Dependent Protein Kinase II Alpha (CAMK2a) ELISA Kit
The Human Calcium/Calmodulin-dependent Protein Kinase II Alpha (CAMK2A) ELISA Kit is specifically designed for the accurate detection and quantification of CAMK2A levels in human serum, plasma, and cell culture supernatants. This kit provides high sensitivity and specificity, ensuring reliable and reproducible results for a variety of research applications.CAMK2A is an important enzyme involved in various cellular processes, including neuronal signaling, synaptic plasticity, and memory formation.
Dysregulation of CAMK2A has been linked to neurological disorders, such as Alzheimer's disease and epilepsy, highlighting its significance as a potential biomarker for studying these conditions and developing new therapeutic strategies.By using the Human CAMK2A ELISA Kit, researchers can gain valuable insights into the role of CAMK2A in disease pathogenesis and potential therapeutic interventions. Get accurate and reliable results with this advanced ELISA kit from Assay Genie.
Product Name:
Human Calcium/Calmodulin Dependent Protein Kinase II Alpha (CAMK2a) ELISA Kit
Product Code:
HUDL00417
Size:
96 Assays
Target:
CAMK2a
Synonyms:
CAMKA
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
83-100
91
EDTA plasma (n=5)
90-103
96
Heparin plasma (n=5)
90-99
94
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
