Human Cadherin EGF LAG Seven Pass G-Type Receptor 2 (CELSR2) ELISA Kit
The Human Cadherin EGF LAG Seven-pass G-type Receptor 2 (CELSR2) ELISA Kit is a reliable and accurate tool for the detection of CELSR2 levels in human serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit provides researchers with precise and reproducible results for a variety of research applications.CELSR2 is a key receptor involved in cell adhesion and signaling, playing a crucial role in various cellular processes. Dysregulation of CELSR2 has been associated with a range of diseases, including cancer, cardiovascular disorders, and neurological conditions, making it a valuable biomarker for studying and developing potential treatments for these diseases.
With the Human CELSR2 ELISA Kit, researchers can confidently measure CELSR2 levels in human samples, providing valuable insights into its functions and potential implications in disease pathology. Upgrade your research capabilities with this advanced ELISA kit today.
Product Name:
Human Cadherin EGF LAG Seven Pass G-Type Receptor 2 (CELSR2) ELISA Kit
Product Code:
HUDL00532
Size:
96 Assays
Target:
CELSR2
Synonyms:
CDHF10, EGFL2, Flamingo 1, MEGF3, Cadherin family member 10, Epidermal growth factor-like protein 2, Multiple epidermal growth factor-like domains protein 3
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
