The Human CacyBP (Calcyclin Binding Protein) CLIA Kit is specifically designed for the precise measurement of CacyBP levels in human samples including serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for various research purposes.CacyBP is a protein that plays a key role in cell signaling pathways and protein degradation processes, making it a crucial factor in various biological processes.
Dysregulation of CacyBP has been implicated in several diseases such as cancer, cardiovascular disorders, and neurodegenerative conditions, highlighting its importance as a biomarker for studying and potentially treating these ailments.With the Human CacyBP CLIA Kit, researchers can confidently measure CacyBP levels in human samples with precision and reliability, enabling further insights into its role in disease pathogenesis and therapeutic development.
Product Name:
Human CACYBP (Calcyclin Binding Protein) CLIA Kit
SKU:
HUES00410
Target:
Human CACYBP (Calcyclin Binding Protein)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human CACYBP. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CACYBP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CACYBP, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human CACYBP. You can calculate the concentration of Human CACYBP in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
103-117
86-100
103-118
Average (%)
109
93
110
1:4
Range (%)
93-107
87-98
84-98
Average (%)
98
92
91
1:8
Range (%)
96-107
104-118
84-98
Average (%)
102
110
91
1:16
Range (%)
92-104
101-113
85-98
Average (%)
99
107
90
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-107
100
EDTA plasma (n=5)
99-114
105
Cell culture media (n=5)
93-105
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
50.04
130.84
357.68
52.13
142.16
351.7
Standard deviation
5.54
14.17
22.14
5.95
12.69
34.33
C V (%)
11.07
10.83
6.19
11.41
8.93
9.76
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human CACYBP concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human CACYBP in samples. No significant cross-reactivity or interference between Human CACYBP and analogues was observed.
