Human CA12 Recombinant Protein (RPPB1465)

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SKU:
RPPB1465
Product type:
Recombinant Protein
Size:
10ug
Species:
Human
Target:
CA12
Synonyms:
Carbonic anhydrase 12
Synonyms:
Carbonate dehydratase XII
Synonyms:
Carbonic anhydrase XII
Synonyms:
CA-XII
Source:
Sf9 Insect cells
Uniprot:
O43570
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Description

Product Name:Human CA12 Recombinant Protein
Product Code:RPPB1465
Size:10µg
Species:Human
Target:CA12
Synonyms:Carbonic anhydrase 12, Carbonate dehydratase XII, Carbonic anhydrase XII, CA-XII, Tumor antigen HOM-RCC-3.1.3, CA12, Carbonic Anhydrase XII, Carbonic anhydrase 12 isoform 1, CAXII, HsT18816.
Source:Sf9 Insect cells
Physical Appearance:Sterile Filtered clear solution.
Formulation:CA12 protein solution (0.5mg/ml) containing Phosphate Buffer Saline (pH7.4) and 10% glycerol.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 90.0% as determined by SDS-PAGE.
Amino Acid Sequence:APVNGSKWTY FGPDGENSWS KKYPSCGGLL QSPIDLHSDI LQYDASLTPL EFQGYNLSAN KQFLLTNNGH SVKLNLPSDM HIQGLQSRYS ATQLHLHWGN PNDPHGSEHT VSGQHFAAEL HIVHYNSDLY PDASTASNKS EGLAVLAVLI EMGSFNPSYD KIFSHLQHVK YKGQEAFVPG FNIEELLPER TAEYYRYRGS LTTPPCNPTV LWTVFRNPVQ ISQEQLLALE TALYCTHMDD PSPREMINNF RQVQKFDERL VYTSFSQVQV CTAAGLSHHH HHH
Biological Activity:Specific activity is > 300 pmol/min/ug, and is defined as the amount of enzyme that hydrolyze 1.0 pmole of 4-nitrophenyl acetate to 4-nitrophenol per minute at pH 7.5 at 37C.

Carbonic anhydrase 12 (CA12) is an enzyme that belongs to the Carbonic anhydrases (CAs) family. This is a large family of zinc metalloenzymes which catalyze the reversible hydration of carbon dioxide. They are involved in various biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. CA12 is a type I membrane protein which is highly expressed in normal tissues, such as the kidney, colon and pancreas, and is overexpressed in 10% of clear cell renal carcinomas.

CA12 Human Recombinant produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 283 amino acids (25-301a.a.) and having a molecular mass of 31.94kDa (Migrates at 28-40kDa on SDS-PAGE under reducing conditions).CA12 is fused to a 6 amino acid His-tag at C-terminus & purified by proprietary chromatographic techniques.

UniProt Protein Function:Reversible hydration of carbon dioxide.
NCBI Summary:Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. This gene product is a type I membrane protein that is highly expressed in normal tissues, such as kidney, colon and pancreas, and has been found to be overexpressed in 10% of clear cell renal carcinomas. Three transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq, Jun 2014]
UniProt Code:O43570
NCBI GenInfo Identifier:5915866
NCBI Gene ID:771
NCBI Accession:O43570.1
UniProt Secondary Accession:O43570,Q53YE5, Q9BWG2, B2RE24,
UniProt Related Accession:O43570
Molecular Weight:38,408 Da
NCBI Full Name:Carbonic anhydrase 12
NCBI Synonym Full Names:carbonic anhydrase 12
NCBI Official Symbol:CA12  
NCBI Official Synonym Symbols:CAXII; CA-XII; T18816; HsT18816  
NCBI Protein Information:carbonic anhydrase 12
UniProt Protein Name:Carbonic anhydrase 12
UniProt Synonym Protein Names:Carbonate dehydratase XII; Carbonic anhydrase XII; CA-XII; Tumor antigen HOM-RCC-3.1.3
Protein Family:Carbonic anhydrase
UniProt Gene Name:CA12  
UniProt Entry Name:CAH12_HUMAN
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