The Human CA1 (Carbonic Anhydrase I) ELISA Kit is specifically designed for the precise quantification of carbonic anhydrase I levels in human samples such as serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit ensures accurate and reproducible results, making it suitable for a variety of research applications.Carbonic anhydrase I is an enzyme that plays a vital role in catalyzing the reversible hydration of carbon dioxide, essential for various physiological processes including acid-base balance, bone resorption, and formation of aqueous humor in the eye.
Dysregulation of carbonic anhydrase I has been associated with diseases such as osteoporosis, glaucoma, and renal tubular acidosis, highlighting its significance as a potential biomarker for studying these conditions and developing therapeutic interventions.
Product Name:
Human CA1 (Carbonic Anhydrase I) ELISA Kit
SKU:
HUES01802
Target:
Human CA1 (Carbonic Anhydrase I)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
46.88 pg/mL
Detection range:
78.13-5000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human CA1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CA1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CA1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human CA1. You can calculate the concentration of Human CA1 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
90-103
93-106
87-99
Average (%)
95
99
92
1:4
Range (%)
90-103
81-94
84-94
Average (%)
97
86
89
1:8
Range (%)
89-103
84-99
81-95
Average (%)
96
90
88
1:16
Range (%)
88-100
84-95
88-101
Average (%)
94
90
95
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-101
94
EDTA plasma (n=5)
90-103
96
Cell culture media (n=5)
85-97
91
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
249.22
743.2
1676.01
254.87
709.81
1747.8
Standard deviation
15.43
44.44
56.15
14.6
31.23
77.25
C V (%)
6.19
5.98
3.35
5.73
4.4
4.42
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human CA1 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human CA1 in samples. No significant cross-reactivity or interference between Human CA1 and analogues was observed.
