The Human CA1 (Carbonic Anhydrase I) CLIA Kit is specifically designed for the quantitative measurement of CA1 levels in human serum, plasma, and cell culture supernatants. This kit offers excellent sensitivity and specificity, allowing for precise and consistent results, making it suitable for various research applications.CA1, also known as Carbonic Anhydrase I, is an enzyme involved in catalyzing the interconversion of carbon dioxide and water to bicarbonate and protons.
It plays a crucial role in maintaining acid-base balance and is implicated in various diseases such as osteoporosis, glaucoma, and renal disorders, making it a valuable biomarker for studying these conditions and potentially developing targeted therapies.Overall, the Human CA1 (Carbonic Anhydrase I) CLIA Kit provides researchers with a reliable tool for accurately measuring CA1 levels, furthering our understanding of its physiological and pathological roles.
Product Name:
Human CA1 (Carbonic Anhydrase I) CLIA Kit
SKU:
HUES00423
Target:
Human CA1 (Carbonic Anhydrase I)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human CA1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CA1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CA1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human CA1. You can calculate the concentration of Human CA1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
85-98
90-103
95-111
Average (%)
91
95
102
1:4
Range (%)
95-107
97-112
93-107
Average (%)
102
104
98
1:8
Range (%)
103-116
95-109
99-115
Average (%)
110
100
107
1:16
Range (%)
96-108
98-115
91-105
Average (%)
102
105
98
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
85-101
92
EDTA plasma (n=5)
92-106
98
Cell culture media (n=5)
97-113
104
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
1.02
1.75
6.92
0.95
1.61
6.71
Standard deviation
0.12
0.13
0.77
0.12
0.14
0.65
C V (%)
11.76
7.43
11.13
12.63
8.7
9.69
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human CA1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human CA1 in samples. No significant cross-reactivity or interference between Human CA1 and analogues was observed.
