The Human C5 Complement Component 5 ELISA Kit is specifically developed for the precise measurement of C5 levels in human samples including serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit ensures accurate and reproducible results, making it suitable for a variety of research purposes.The C5 complement component plays a critical role in the innate immune response, contributing to the body's defense against pathogens and foreign invaders.
Dysregulation of C5 has been linked to various inflammatory and autoimmune diseases, making it a valuable biomarker for studying these conditions and exploring potential therapeutic interventions.Overall, the Human C5 Complement Component 5 ELISA Kit provides researchers with a reliable tool for investigating the role of C5 in health and disease, offering valuable insights into immune system function and potential treatment strategies.
Product Name:
Human C5 (Complement Component 5) ELISA Kit
SKU:
HUES01946
Target:
Human C5 (Complement Component 5)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
46.88 pg/mL
Detection range:
78.13-5000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human C5. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human C5 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human C5, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human C5. You can calculate the concentration of Human C5 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
97-112
97-113
92-107
Average (%)
103
105
99
1:4
Range (%)
93-107
86-99
87-99
Average (%)
99
92
92
1:8
Range (%)
90-101
85-96
82-96
Average (%)
95
90
89
1:16
Range (%)
88-104
82-95
83-94
Average (%)
95
87
88
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-100
95
EDTA plasma (n=5)
95-108
100
Cell culture media (n=5)
93-106
100
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
253.77
643.02
2124.74
257.27
684.79
2226.57
Standard deviation
15.18
30.8
102.41
15.51
38.42
98.86
C V (%)
5.98
4.79
4.82
6.03
5.61
4.44
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human C5 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human C5 in samples. No significant cross-reactivity or interference between Human C5 and analogues was observed.
