The Human C4a Complement Component 4a ELISA Kit is a highly reliable and accurate tool for detecting levels of C4a complement component 4a in human serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring precise and reproducible results for a variety of research applications.C4a complement component 4a is a key protein involved in the immune system's complement pathway, playing a crucial role in inflammation and immune response. Abnormal levels of C4a have been linked to various autoimmune diseases, inflammatory conditions, and infectious diseases, making it a valuable biomarker for studying these conditions and developing potential therapies.
With its advanced technology and optimized protocols, the Human C4a Complement Component 4a ELISA Kit is a valuable tool for researchers seeking to investigate the role of C4a in disease pathogenesis and therapeutic interventions. Trust in this kit for accurate and reliable results in your research endeavors.
Product Name:
Human C4a (Complement Component 4a) ELISA Kit
SKU:
HUES01456
Target:
Human C4a (Complement Component 4a)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.94 ng/mL
Detection range:
1.56-100 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human C4a. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human C4a and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human C4a, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human C4a. You can calculate the concentration of Human C4a in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
91-103
98-113
87-99
Average (%)
98
104
94
1:4
Range (%)
91-102
87-101
85-98
Average (%)
96
93
91
1:8
Range (%)
93-105
84-96
86-96
Average (%)
98
88
91
1:16
Range (%)
89-104
82-92
87-99
Average (%)
95
87
94
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-100
95
EDTA plasma (n=5)
86-101
92
Cell culture media (n=5)
91-106
97
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
4.84
13.18
42.91
4.58
13.63
39.0
Standard deviation
0.27
0.7
1.77
0.23
0.75
1.56
C V (%)
5.58
5.31
4.12
5.02
5.5
4.0
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human C4a concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human C4a in samples. No significant cross-reactivity or interference between Human C4a and analogues was observed.
