The Human C3a Complement Component 3a ELISA Kit is a valuable tool for researchers looking to accurately measure levels of the C3a complement component in human samples. This kit offers high sensitivity and specificity, ensuring precise and consistent results for serum, plasma, and cell culture supernatants. The C3a complement component is a key player in the immune system, playing a critical role in inflammation and immune response. Abnormal levels of C3a have been linked to various autoimmune diseases, infections, and inflammatory conditions, making it a crucial biomarker for understanding and studying these diseases.
With easy-to-follow protocols and reliable performance, the Human C3a Complement Component 3a ELISA Kit is essential for researchers focused on immunology, inflammation, and disease mechanisms. This kit provides a convenient and accurate solution for measuring C3a levels in human samples, aiding in the advancement of research and potential therapeutic developments.
Product Name:
Human C3a (Complement Component 3a) ELISA Kit
SKU:
HUES01954
Target:
Human C3a (Complement Component 3a)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human C3a. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human C3a and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human C3a, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human C3a. You can calculate the concentration of Human C3a in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
89-107
91-107
87-103
Average (%)
97
98
95
1:4
Range (%)
93-105
81-94
85-97
Average (%)
99
87
90
1:8
Range (%)
91-103
82-92
86-97
Average (%)
97
87
92
1:16
Range (%)
89-106
85-96
84-96
Average (%)
97
91
91
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
84-97
90
EDTA plasma (n=5)
91-102
97
Cell culture media (n=5)
87-98
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
1.01
2.09
8.39
0.94
2.22
7.83
Standard deviation
0.07
0.09
0.3
0.05
0.13
0.24
C V (%)
6.93
4.31
3.58
5.32
5.86
3.07
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human C3a concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human C3a in samples. No significant cross-reactivity or interference between Human C3a and analogues was observed.
