Human C1QTNF3 (C1q and Tumor Necrosis Factor Related Protein 3) ELISA Kit
The Human C1QTNF3 (C1q and Tumor Necrosis Factor-Related Protein 3) ELISA Kit is specifically designed for the accurate measurement of C1QTNF3 levels in human serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reliable results for a variety of research purposes.C1QTNF3, also known as CTRP3, is a protein that belongs to the C1q/TNF-related protein family and plays a crucial role in regulating metabolism, inflammation, and insulin sensitivity.
Dysregulation of C1QTNF3 has been linked to various metabolic disorders, cardiovascular diseases, and inflammatory conditions, making it a valuable biomarker for understanding these disorders and developing potential therapeutic interventions.With its advanced technology and performance, the Human C1QTNF3 ELISA Kit is an essential tool for researchers studying the role of C1QTNF3 in various diseases and exploring its potential as a target for novel therapeutic strategies.
Product Name:
Human C1QTNF3 (C1q and Tumor Necrosis Factor Related Protein 3) ELISA Kit
SKU:
HUES03502
Target:
Human C1QTNF3 (C1q and Tumor Necrosis Factor Related Protein 3)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.38 ng/mL
Detection range:
0.63-40 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human C1QTNF3. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human C1QTNF3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human C1QTNF3, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human C1QTNF3. You can calculate the concentration of Human C1QTNF3 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
88-104
96-107
90-101
Average (%)
95
102
95
1:4
Range (%)
90-104
82-93
82-95
Average (%)
96
87
88
1:8
Range (%)
86-101
85-98
84-99
Average (%)
93
90
90
1:16
Range (%)
94-109
87-101
84-95
Average (%)
99
92
90
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-106
98
EDTA plasma (n=5)
87-98
93
Cell culture media (n=5)
91-105
97
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
2.12
6.17
13.89
1.96
6.09
14.78
Standard deviation
0.11
0.31
0.51
0.13
0.27
0.81
C V (%)
5.19
5.02
3.67
6.63
4.43
5.48
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human C1QTNF3 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human C1QTNF3 in samples. No significant cross-reactivity or interference between Human C1QTNF3 and analogues was observed.
