Human C-Type Lectin Domain Family 3, Member B (CLEC3B) ELISA Kit
The Human CLEC3B (C-Type Lectin Domain Family 3 Member B) ELISA Kit is a powerful tool for the precise measurement of CLEC3B levels in human samples, including serum, plasma, and cell culture supernatants. With its superior sensitivity and specificity, this kit delivers accurate and consistent results, making it suitable for a wide range of research applications.CLEC3B, also known as tetranectin, is a key protein involved in various biological processes, including cell adhesion, angiogenesis, and tissue remodeling.
Its dysregulation has been implicated in diseases such as cancer, inflammatory disorders, and cardiovascular diseases, underscoring its significance as a potential therapeutic target and diagnostic biomarker.By utilizing the Human CLEC3B ELISA Kit, researchers can gain valuable insights into the role of CLEC3B in health and disease, paving the way for new discoveries and treatment strategies. Order your kit today and unlock the potential of CLEC3B in your research endeavors.
Product Name:
Human C-Type Lectin Domain Family 3, Member B (CLEC3B) ELISA Kit
Product Code:
HUDL00594
Size:
96 Assays
Target:
CLEC3B
Synonyms:
TN, TNA, Tetranectin, Plasminogen Binding Protein, Plasminogen kringle 4-binding protein
Detection Method:
Sandwich
Range:
0.187-12ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
83-100
91
EDTA plasma (n=5)
91-98
94
Heparin plasma (n=5)
90-99
94
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
