The Human BSP (Bone Sialoprotein) ELISA Kit is a reliable and accurate tool for the detection of bone sialoprotein levels in human samples, including serum, plasma, and cell culture supernatants. With high sensitivity and specificity, this kit provides consistent and reproducible results, making it suitable for a variety of research applications.Bone sialoprotein is a key protein involved in bone mineralization and remodeling, making it a valuable biomarker for studying bone health and diseases such as osteoporosis and bone metastasis.
The Human BSP ELISA Kit is essential for researchers and healthcare professionals looking to understand the role of bone sialoprotein in various physiological and pathological processes, as well as for developing potential therapeutics targeting bone-related conditions.
Product Name:
Human BSP (Bone Sialoprotein) ELISA Kit
SKU:
HUES01764
Target:
Human BSP (Bone Sialoprotein)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.38 ng/mL
Detection range:
0.63-40 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human IBSP. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human IBSP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IBSP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human IBSP. You can calculate the concentration of Human IBSP in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
89-101
85-96
92-107
Average (%)
95
91
98
1:4
Range (%)
92-110
83-94
82-94
Average (%)
100
88
88
1:8
Range (%)
87-99
79-92
89-99
Average (%)
94
85
94
1:16
Range (%)
89-103
85-98
81-95
Average (%)
94
90
87
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-106
100
EDTA plasma (n=5)
95-107
101
Cell culture media (n=5)
87-100
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
2.11
4.21
17.02
1.92
4.39
15.44
Standard deviation
0.12
0.2
0.58
0.13
0.21
0.74
C V (%)
5.69
4.75
3.41
6.77
4.78
4.79
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human IBSP concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human IBSP in samples. No significant cross-reactivity or interference between Human IBSP and analogues was observed.
