Human Breast And Kidney Expressed Chemokine (BRAK) ELISA Kit
The Human Breast and Kidney Expressed Chemokine (BRAK) ELISA Kit is a powerful tool designed for the accurate quantification of BRAK levels in human samples such as serum, plasma, and cell culture supernatants. With exceptional sensitivity and specificity, this kit delivers robust and reproducible results, making it well-suited for a wide range of research applications.BRAK, also known as CXCL14, is a chemokine that plays a critical role in immune response modulation, inflammation, and tumorigenesis. Its expression in breast and kidney tissues suggests its involvement in various physiological and pathological processes in these organs.
By measuring BRAK levels, researchers can gain valuable insights into the role of this chemokine in cancer, inflammation, and other diseases, potentially leading to the development of novel therapeutic strategies.With the Human Breast and Kidney Expressed Chemokine (BRAK) ELISA Kit, researchers can explore the intricate functions of BRAK and its implications in human health and disease, paving the way for advancements in biomedical research and clinical diagnostics.
Product Name:
Human Breast And Kidney Expressed Chemokine (BRAK) ELISA Kit
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
