Human Bone Morphogenetic Protein Receptor 1B (BMPR1B) ELISA Kit
The Human Bone Morphogenetic Protein Receptor 1B (BMPR1B) ELISA Kit is a valuable tool for the precise measurement of BMPR1B levels in human samples such as serum, plasma, and cell culture supernatants. This kit is known for its exceptional sensitivity and specificity, ensuring accurate and consistent results for a variety of research purposes.BMPR1B is a crucial receptor involved in the BMP signaling pathway, which plays a significant role in bone formation, cell differentiation, and embryonic development. Dysregulation of BMPR1B signaling has been implicated in various diseases, including skeletal disorders and certain types of cancer, highlighting its importance as a potential therapeutic target.
By using the Human BMPR1B ELISA Kit, researchers can easily and effectively quantify BMPR1B levels in human samples, helping to better understand its biological functions and potential implications in disease pathogenesis. This kit is an indispensable tool for studying BMPR1B-related mechanisms and developing novel treatment strategies.
Product Name:
Human Bone Morphogenetic Protein Receptor 1B (BMPR1B) ELISA Kit
Product Code:
HUDL00349
Size:
96 Assays
Target:
BMPR1B
Synonyms:
CDw293, CD293, BMPR1-B, ALK6
Detection Method:
Sandwich
Range:
0.312-20ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
83-100
91
EDTA plasma (n=5)
90-103
96
Heparin plasma (n=5)
89-102
95
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
