Human BNP (27-102) Recombinant Protein (RPPB0115)
- SKU:
- RPPB0115
- Product type:
- Recombinant Protein
- Size:
- 10ug
- Species:
- Human
- Target:
- BNP (27-102)
- Synonyms:
- NPPB
- Natriuretic Peptide Precursor B
- BNP
- B-type Natriuretic Peptide
- Source:
- Escherichia Coli
- Uniprot:
- P16860
Description
| Product Name: | Human BNP (27-102) Recombinant Protein |
| Product Code: | RPPB0115 |
| Size: | 10µg |
| Species: | Human |
| Target: | BNP (27-102) |
| Synonyms: | NPPB, Natriuretic Peptide Precursor B, BNP, B-type Natriuretic Peptide. |
| Source: | Escherichia Coli |
| Physical Appearance: | Filtered White lyophilized (freeze-dried) powder. |
| Formulation: | BNP filtered (0.4 µm) and lyophilized from 0.5mg/ml in 20 mM Tris buffer and 50 mM NaCl, pH 7.5. |
| Solubility: | It is recommended to add 200µl deionized water to prepare a working stock solution of approximately 0.5mg/ml and let the lyophilized pellet dissolve completely. BNP is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture. |
| Stability: | Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C. |
| Purity: | Greater than 95.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MKHHHHHHPL GSPGSASDLE TSGLQEQRNH LQGKLSELQV EQTSLEPLQE SPRPTGVWKS REVATEGIRG HRKMVLYTLR APR |
Natriuretic Peptide Precursor B acts as a cardiac hormone with a variety of biological actions including natriuresis, diuresis, vasorelaxation, and inhibition of renin and aldosterone secretion. It is thought to play a key role in cardiovascular homeostasis. Helps restore the body's salt and water balance. Improves heart function.
BNP Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain (a.a 27-102) containing 83 amino acids including a 7 a.a N-terminal His tag. The total molecular mass is 9.4kDa (calculated).
| UniProt Protein Function: | NPPB: Cardiac hormone which may function as a paracrine antifibrotic factor in the heart. Also plays a key role in cardiovascular homeostasis through natriuresis, diuresis, vasorelaxation, and inhibition of renin and aldosterone secretion. Specifically binds and stimulates the cGMP production of the NPR1 receptor. Binds the clearance receptor NPR3. Belongs to the natriuretic peptide family. |
| UniProt Protein Details: | Protein type:Secreted; Secreted, signal peptide; Hormone Chromosomal Location of Human Ortholog: 1p36.2 Cellular Component: extracellular region; extracellular space; protein complex Molecular Function:diuretic hormone activity; hormone activity; protein binding; receptor binding Biological Process: body fluid secretion; cell surface receptor linked signal transduction; cGMP biosynthetic process; negative regulation of angiogenesis; negative regulation of cell growth; receptor guanylyl cyclase signaling pathway; regulation of blood pressure; regulation of vascular permeability; regulation of vasodilation |
| NCBI Summary: | This gene is a member of the natriuretic peptide family and encodes a secreted protein which functions as a cardiac hormone. The protein undergoes two cleavage events, one within the cell and a second after secretion into the blood. The protein's biological actions include natriuresis, diuresis, vasorelaxation, inhibition of renin and aldosterone secretion, and a key role in cardiovascular homeostasis. A high concentration of this protein in the bloodstream is indicative of heart failure. The protein also acts as an antimicrobial peptide with antibacterial and antifungal activity. Mutations in this gene have been associated with postmenopausal osteoporosis. [provided by RefSeq, Nov 2014] |
| UniProt Code: | P16860 |
| NCBI GenInfo Identifier: | 113836 |
| NCBI Gene ID: | 4879 |
| NCBI Accession: | P16860.1 |
| UniProt Secondary Accession: | P16860,Q6FGY0, Q9P2Q7, B0ZBE9, |
| UniProt Related Accession: | P16860 |
| Molecular Weight: | 14,726 Da |
| NCBI Full Name: | Natriuretic peptides B |
| NCBI Synonym Full Names: | natriuretic peptide B |
| NCBI Official Symbol: | NPPB |
| NCBI Official Synonym Symbols: | BNP |
| NCBI Protein Information: | natriuretic peptides B |
| UniProt Protein Name: | Natriuretic peptides B |
| UniProt Synonym Protein Names: | Gamma-brain natriuretic peptide |
| Protein Family: | Brain natriuretic peptide |
| UniProt Gene Name: | NPPB |
| UniProt Entry Name: | ANFB_HUMAN |
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