The Human Biliverdin Reductase B (BLVRB) ELISA Kit is a powerful tool for the precise measurement of BLVRB levels in human biological samples such as serum, plasma, and cell culture supernatants. With exceptional sensitivity and specificity, this kit delivers consistent and accurate results, making it an essential resource for a wide variety of research endeavors.BLVRB is a vital enzyme involved in the conversion of biliverdin to bilirubin, a process critical for the breakdown of heme and the regulation of oxidative stress.
Dysregulation of BLVRB has been associated with various diseases, including liver disorders, cardiovascular conditions, and neurodegenerative illnesses, thus highlighting its clinical significance as a potential biomarker for disease diagnosis and therapy development.Don't miss out on the opportunity to advance your research with the Human Biliverdin Reductase B (BLVRB) ELISA Kit, a reliable and effective solution for studying BLVRB levels in human samples.
Product Name:
Human Biliverdin Reductase B (BLVRB) ELISA Kit
Product Code:
HUDL00337
Size:
96 Assays
Target:
BLVRB
Synonyms:
FLR, SDR43U1, Flavin Reductase,NADPH, Short Chain Dehydrogenase/Reductase Family 43U,Member 1, Biliverdin-IX beta-reductase, Green heme-binding protein
Detection Method:
Sandwich
Range:
0.312-20ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
