Human BID Recombinant Protein (RPPB2889)
- SKU:
- RPPB2889
- Product type:
- Recombinant Protein
- Size:
- 10ug
- Species:
- Human
- Target:
- BID
- Synonyms:
- BH3-interacting domain death agonist
- p22 BID
- BID
- FP497
- Source:
- Escherichia Coli
- Uniprot:
- P55957
Description
Product Name: | Human BID Recombinant Protein |
Product Code: | RPPB2889 |
Size: | 10µg |
Species: | Human |
Target: | BID |
Synonyms: | BH3-interacting domain death agonist, p22 BID, BID, FP497, MGC15319, MGC42355. |
Source: | Escherichia Coli |
Physical Appearance: | Sterile Filtered colorless solution. |
Formulation: | The protein solution contains 20mM Tris-HCl pH-8 & 20% NaCl. |
Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
Purity: | Greater than 95.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE. |
Amino Acid Sequence: | MDCEVNNGSS LRDECITNLL VFGFLQSCSD NSFRRELDAL GHELPVLAPQ WEGYDELQTD GNRSSHSRLG RIEADSESQE DIIRNIARHL AQVGDSMDRS IPPGLVNGLA LQLRNTSRSE EDRNRDLATA LEQLLQAYPR DMEKEKTMLV LALLLAKKVA SHTPSLLRDV FHTTVNFINQ NLRTYVRSLA RNGMD |
BID accession number NP_001187 is a pro-apoptotic Bcl-2 protein having only the BH3 domain. In reaction to apoptotic signaling, BID interacts with another Bcl-2 family of cell death regulators, called Bax, they form a heterodimer resulting to the insertion of Bax into the outer mitochondrial membrane. Bax induces the opening of the mitochondrial voltage-dependent anion channel which lead to the release of cytochrome c and other pro-apoptotic factors from the mitochondria resulting in activation of caspases. BID is a mediator of mitochondrial damage induced by caspase-8 (CASP8). CASP8 cleaves BID, and the COOH-terminal part translocates to mitochondria where it triggers cytochrome c release. The major proteolytic product p15 BID releasea cytochrome c. Isoform 1, Isoform 2 and Isoform 4 induce ice-like proteases and apoptosis while Isoform 3 does not induce apoptosis.
BID Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 195 amino acids and having a molecular mass of 21.9 kDa.
UniProt Protein Function: | BID: a pro-apoptotic member of the Bcl-2 superfamily. Targets intracellular membranes and contains a BH3 death domain. Heterodimerizes with either the pro-apoptotic protein BAX or the anti-apoptotic protein BCL2, antagonizing its protective effect. The activity of BID is regulated by Caspase 8-mediated cleavage, exposing the BH3 domain and significantly changing the surface charge and hydrophobicity, causing translocation to mitochondria where it triggers cytochrome c release. Multiple alternatively spliced variants have been found. |
UniProt Protein Details: | Protein type:Mitochondrial; Apoptosis Chromosomal Location of Human Ortholog: 22q11.1 Cellular Component: cytosol; membrane; mitochondrial outer membrane; mitochondrion Molecular Function:death receptor binding; protein binding Biological Process: apoptotic mitochondrial changes; DNA damage response, signal transduction; induction of apoptosis via death domain receptors; neuron apoptosis; positive regulation of apoptosis; positive regulation of protein homooligomerization; positive regulation of protein oligomerization; regulation of apoptosis; release of cytochrome c from mitochondria |
NCBI Summary: | This gene encodes a death agonist that heterodimerizes with either agonist BAX or antagonist BCL2. The encoded protein is a member of the BCL-2 family of cell death regulators. It is a mediator of mitochondrial damage induced by caspase-8 (CASP8); CASP8 cleaves this encoded protein, and the COOH-terminal part translocates to mitochondria where it triggers cytochrome c release. Multiple alternatively spliced transcript variants have been found, but the full-length nature of some variants has not been defined. [provided by RefSeq, Jul 2008] |
UniProt Code: | P55957 |
NCBI GenInfo Identifier: | 2493285 |
NCBI Gene ID: | 637 |
NCBI Accession: | P55957.1 |
UniProt Secondary Accession: | P55957,Q549M7, Q71T04, Q7Z4M9, Q8IY86, |
UniProt Related Accession: | P55957 |
Molecular Weight: | 11,263 Da |
NCBI Full Name: | BH3-interacting domain death agonist |
NCBI Synonym Full Names: | BH3 interacting domain death agonist |
NCBI Official Symbol: | BID |
NCBI Official Synonym Symbols: | FP497 |
NCBI Protein Information: | BH3-interacting domain death agonist |
UniProt Protein Name: | BH3-interacting domain death agonist |
UniProt Synonym Protein Names: | p22 BID; BID |
Protein Family: | BH3-interacting domain death agonist |
UniProt Gene Name: | BID |
UniProt Entry Name: | BID_HUMAN |