The BH3 interacting domain death agonist (BID) is a pro-apoptotic member of the Bcl-2 protein family; which contains only the BH3 domain; and is required for its interaction with the Bcl-2 family proteins and for its pro-death activity. BID is important to cell death mediated by these proteases and thus is the sentinel to protease-mediated death signals. Recent studies further indicate that Bid may be more than just a killer molecule; it could be also involved in the maintenance of genomic stability by engaging at mitosis checkpoint. BID is an integrating key regulator of the intrinsic death pathway that amplifies caspase-dependent and caspase-independent execution of neuronal apoptosis. Therefore pharmacological inhibition of BID provides a promising therapeutic strategy in neurological diseases where programmed cell death is prominent. BID is activated by Caspase 8 in response to Fas/TNF-R1 death receptor activation. Activated BID is translocated to mitochondria and induces cytochrome c release; which in turn activates downstream caspases. BID action has been proposed to involve the mitochondrial re-location of its truncated form; tBid; to facilitate the release of apoptogenic proteins like cytochrome c.
Product Name:
Human BID Recombinant Protein (RPES2923)
Product Code:
RPES2923
Size:
50µg
Species:
Human
Expressed Host:
E.coli
Synonyms:
BH3-Interacting Domain Death Agonist, p22 BID, BID
Accession:
P55957-1
Sequence:
Met 1-Asp 195
Activity:
1. Measured by its binding ability in a functional ELISA.2. Immobilized human BID at 10 µg/mL (100 ?l/well) can bind biotinylated human BCL2L1; The EC50 of biotinylated human BCL2L1 is 7.1 ng/mL.3. Immobilized human BID at 10 µg/mL (100 ?l/well) can bind biotinylated mouse BCL2L1; The EC50 of biotinylated mouse BCL2L1 is 5.6 ng/mL.
Endotoxin:
Please contact us for more information.
Protein Construction:
A DNA sequence encoding the human BID isoform 1 (P55957-1) (Met 1-Asp 195) was expressed and purified; with additional two amino acids (Gly & Pro) at the N-terminus.
Purity:
> 90 % as determined by reducing SDS-PAGE.
AP Mol Mass:
22 kDa
Formulation:
Lyophilized from sterile 40mM Tris; 150mM NaCl; pH 8.0
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
