Human Beta-NGF/NGFB Recombinant Protein (RPES0265)

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SKU:
RPES0265
Product type:
Recombinant Protein
Host species:
E.coli
Reactivity:
Human
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Description

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Human Beta-NGF/NGFB Recombinant Protein

The precursor form of the nerve growth factor (proNGF) like its mature form is characterized by the cystin knot motif consisting of three cystine bridges; whereas proneurotrophins and mature neurotrophins elicit opposite biological effects. ProNGF functions preferentially via the complex of pan-neurotrophin receptor p75 (p75NTR) and vps10p domain-containing receptor sortilin inducing neuronal apoptosis and contributing to age- and disease-related neurodegeneration.

Product Name:Human Beta-NGF/NGFB Recombinant Protein (RPES0265)
Product Code:RPES0265
Size:10µg
Species:Human
Expressed Host:E.coli
Synonyms:Beta-Nerve Growth Factor, Beta-NGF, NGF, NGFB
Accession:P01138
Sequence:Glu19-Ala241
Activity:Measured in a cell proliferation assay using TF?1 human erythroleukemic cells. The ED50 for this effect is 32697 pg/mL.
Endotoxin:<1.0 EU per µg as determined by the LAL method.
Protein Construction:Recombinant Human pro-Nerve Growth Factor is produced by our E.coli expression system and the target gene encoding Glu19-Ala241 is expressed.
Purity:> 95% as determined by reducing SDS-PAGE.
Mol Mass:25 kDa
AP Mol Mass:30 kDa
Formulation:Lyophilized from a 0.2 µm filtered solution of 20mM PB; 250mM NaCl; pH 7.2.
Shipping:This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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