Human Beta-1,3-Glucuronyltransferase 1 (b3GAT1) ELISA Kit

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SKU:
HUDL00300
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q9P2W7
Sensitivity:
0.053ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
CD57,B3-GAT1,GLCATP,GlcAT-P,GlcUAT-P,HNK-1,LEU7,NK-1,Glucuronosyltransferase P,Galactosylgalactosylxylosylprotein 3-Beta-Glucuronosyltransferase 1
Reactivity:
Human
Sample Type:
Tissue Homogenates, Cell Lysates And Other Biological Fluids
Frequently bought together:

Description

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Human Beta-1,3-Glucuronyltransferase 1 (b3GAT1) ELISA Kit

The Human Beta-1,3-Glucuronyltransferase 1 (B3GAT1) ELISA Kit is a valuable tool for the accurate and quantitative measurement of B3GAT1 levels in human biological samples such as serum, plasma, and cell culture supernatants. This kit is known for its high sensitivity and specificity, ensuring reliable and reproducible results for a variety of research applications.B3GAT1 is an important enzyme involved in the biosynthesis of proteoglycans, which play a crucial role in cell signaling, cell adhesion, and extracellular matrix organization.

Dysregulation of B3GAT1 expression has been linked to various human diseases, including developmental disorders, cancer, and inflammatory conditions. Therefore, the Human B3GAT1 ELISA Kit provides researchers with a valuable tool for studying the role of B3GAT1 in disease pathogenesis and identifying potential therapeutic targets.

Product Name:Human Beta-1,3-Glucuronyltransferase 1 (b3GAT1) ELISA Kit
Product Code:HUDL00300
Size:96 Assays
Target:b3GAT1
Synonyms:CD57, B3-GAT1, GLCATP, GlcAT-P, GlcUAT-P, HNK-1, LEU7, NK-1, Glucuronosyltransferase P, Galactosylgalactosylxylosylprotein 3-Beta-Glucuronosyltransferase 1
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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