Human BDNF (Brain Derived Neurotrophic Factor) ELISA Kit
The Human BDNF (Brain Derived Neurotrophic Factor) ELISA Kit is specifically designed for the precise measurement of BDNF levels in human samples including serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit ensures accurate and dependable results, making it well-suited for a wide range of research applications.BDNF is a key neurotrophic factor that plays a crucial role in the growth, survival, and function of neurons in the brain. Dysregulation of BDNF has been associated with various neurological and psychiatric disorders such as depression, Alzheimer's disease, and schizophrenia.
Therefore, the detection and quantification of BDNF levels can provide valuable insights into the pathophysiology of these conditions and aid in the development of targeted treatment strategies.Overall, the Human BDNF ELISA Kit from Assaygenie is an essential tool for researchers seeking to study the role of BDNF in neurological disorders and explore potential therapeutic interventions.
Product Name:
Human BDNF (Brain Derived Neurotrophic Factor) ELISA Kit
SKU:
HUES01297
Target:
Human BDNF (Brain Derived Neurotrophic Factor)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human BDNF. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human BDNF and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human BDNF, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human BDNF. You can calculate the concentration of Human BDNF in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
95-111
94-112
94-109
Average (%)
102
102
100
1:4
Range (%)
97-112
84-96
95-106
Average (%)
104
89
101
1:8
Range (%)
100-114
85-99
96-112
Average (%)
106
92
102
1:16
Range (%)
96-109
85-97
92-103
Average (%)
104
90
98
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
96-109
101
EDTA plasma (n=5)
88-100
93
Cell culture media (n=5)
92-106
100
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
105.8
237.4
809.5
114.2
237.5
768.5
Standard deviation
5.8
12.8
33.2
6.7
10.0
28.4
C V (%)
5.48
5.39
4.1
5.87
4.21
3.7
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human BDNF concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human BDNF in samples. No significant cross-reactivity or interference between Human BDNF and analogues was observed.
