Human BDNF (Brain Derived Neurotrophic Factor) CLIA Kit
The Human BDNF (Brain-Derived Neurotrophic Factor) CLIA Kit is specially designed for precise and accurate detection of BDNF levels in human serum, plasma, and cell culture supernatants. This kit boasts high sensitivity and specificity, ensuring dependable and reproducible results, making it an ideal choice for a wide array of research applications.BDNF is a vital neurotrophin that plays a key role in neuronal survival, growth, and differentiation. It is essential for brain function and has been linked to various neurological and psychiatric disorders, including depression, Alzheimer's disease, and schizophrenia.
The detection of BDNF levels can provide valuable insights into these conditions, aiding in their study and the development of potential therapeutic interventions.Overall, the Human BDNF CLIA Kit is a valuable tool for researchers looking to study the role of BDNF in various physiological and pathological processes, offering reliable and accurate measurements for their experimental needs.
Product Name:
Human BDNF (Brain Derived Neurotrophic Factor) CLIA Kit
SKU:
HUES00010
Target:
Human BDNF (Brain Derived Neurotrophic Factor)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
46.88 pg/mL
Detection range:
78.13-5000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human BDNF. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human BDNF and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human BDNF, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human BDNF. You can calculate the concentration of Human BDNF in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
100-114
97-114
92-106
Average (%)
108
105
97
1:4
Range (%)
92-108
98-111
88-100
Average (%)
100
105
95
1:8
Range (%)
99-116
91-106
93-108
Average (%)
107
98
99
1:16
Range (%)
87-103
89-101
88-102
Average (%)
94
95
95
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
95-109
101
EDTA plasma (n=5)
94-112
102
Cell culture media (n=5)
98-111
103
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
275.32
499.95
2371.43
281.93
486.78
2574.49
Standard deviation
25.11
58.84
235.01
26.16
48.09
186.91
C V (%)
9.12
11.77
9.91
9.28
9.88
7.26
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human BDNF concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human BDNF in samples. No significant cross-reactivity or interference between Human BDNF and analogues was observed.
