Human BDH1 Recombinant Protein (RPPB1441)
- SKU:
- RPPB1441
- Product type:
- Recombinant Protein
- Size:
- 5ug
- Species:
- Human
- Target:
- BDH1
- Synonyms:
- D-beta-hydroxybutyrate dehydrogenase
- mitochondrial
- BDH
- 3-hydroxybutyrate dehydrogenase
- Source:
- Escherichia Coli
- Uniprot:
- Q02338
Description
| Product Name: | Human BDH1 Recombinant Protein |
| Product Code: | RPPB1441 |
| Size: | 5µg |
| Species: | Human |
| Target: | BDH1 |
| Synonyms: | D-beta-hydroxybutyrate dehydrogenase, mitochondrial, BDH, 3-hydroxybutyrate dehydrogenase, BDH1, SDR9C1. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | The BDH1 solution (0.25mg/ml) contains 20mM Tris-HCl buffer (pH8.0), 30% glycerol, 0.2M NaCl, 2mM DTT and 0.1mM PMSF. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 85% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MASAAEPVGS KAVLVTGCDS GFGFSLAKHL HSKGFLVFAG CLMKDKGHDG VKELDSLNSD RLRTVQLNVC SSEEVEKVVE IVRSSLKDPE KGMWGLVNNA GISTFGEVEF TSLETYKQVA EVNLWGTVRM TKSFLPLIRR AKGRVVNISS MLGRMANPAR SPYCITKFGV EAFSDCLRYE MYPLGVKVSV VEPGNFIAAT SLYSPESIQA IAKKMWEELP EVVRKDYGKK YFDEKIAKME TYCSSGSTDT SPVIDAVTHA LTATTPYTRY HPMDYYWWLR MQIMTHLPGA ISDMIYIR |
BDH1 belongs to the short-chain dehydrogenase/reductase gene family. BDH1 forms a homotetrameric lipid-requiring enzyme of the mitochondrial membrane and has a specific necessity for phosphatidylcholine for optimal enzymatic activity. BDH1 catalyzes the interconversion of acetoacetate and (R)-3-hydroxybutyrate, the 2 main ketone bodies formed during fatty acid catabolism.
BDH1 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 318 amino acids (47-343) and having a molecular mass of 35.3kDa.BDH1 is fused to a 21 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | BDH: a member of the short-chain dehydrogenase/reductase gene family. The encoded protein forms a homotetrameric lipid-requiring enzyme of the mitochondrial membrane and has a specific requirement for phosphatidylcholine for optimal enzymatic activity. The encoded protein catalyzes the interconversion of acetoacetate and (R)-3-hydroxybutyrate, the two major ketone bodies produced during fatty acid catabolism. Alternatively spliced transcript variants encoding the same protein have been described. [provided by RefSeq, Jul 2008] |
| UniProt Protein Details: | Protein type:Carbohydrate Metabolism - butanoate; Oxidoreductase; EC 1.1.1.30; Lipid Metabolism - synthesis and degradation of ketone bodies; Mitochondrial Chromosomal Location of Human Ortholog: 3q29 Cellular Component: nucleoplasm; mitochondrion; mitochondrial matrix; cytoplasm; mitochondrial inner membrane Molecular Function:3-hydroxybutyrate dehydrogenase activity; phospholipid binding Biological Process: response to drug; response to toxin; cellular lipid metabolic process; ketone body metabolic process; liver development; response to estradiol stimulus; response to insulin stimulus; response to starvation; response to cadmium ion; response to ethanol; ketone body catabolic process; ketone body biosynthetic process; brain development; response to nutrient; response to corticosterone stimulus |
| NCBI Summary: | This gene encodes a member of the short-chain dehydrogenase/reductase gene family. The encoded protein forms a homotetrameric lipid-requiring enzyme of the mitochondrial membrane and has a specific requirement for phosphatidylcholine for optimal enzymatic activity. The encoded protein catalyzes the interconversion of acetoacetate and (R)-3-hydroxybutyrate, the two major ketone bodies produced during fatty acid catabolism. Alternatively spliced transcript variants encoding the same protein have been described. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q02338 |
| NCBI GenInfo Identifier: | 25108876 |
| NCBI Gene ID: | 622 |
| NCBI Accession: | Q02338.3 |
| UniProt Secondary Accession: | Q02338,Q96ET1, Q9BRZ4, D3DXC0, |
| UniProt Related Accession: | Q02338 |
| Molecular Weight: | 343 |
| NCBI Full Name: | D-beta-hydroxybutyrate dehydrogenase, mitochondrial |
| NCBI Synonym Full Names: | 3-hydroxybutyrate dehydrogenase, type 1 |
| NCBI Official Symbol: | BDH1 |
| NCBI Official Synonym Symbols: | BDH; SDR9C1 |
| NCBI Protein Information: | D-beta-hydroxybutyrate dehydrogenase, mitochondrial; (R)-3-hydroxybutyrate dehydrogenase; 3-hydroxybutyrate dehydrogenase (heart, mitochondrial); short chain dehydrogenase/reductase family 9C, member 1 |
| UniProt Protein Name: | D-beta-hydroxybutyrate dehydrogenase, mitochondrial |
| UniProt Synonym Protein Names: | 3-hydroxybutyrate dehydrogenase |
| Protein Family: | (R,R)-butanediol dehydrogenase |
| UniProt Gene Name: | BDH1 |
| UniProt Entry Name: | BDH_HUMAN |
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