Human Bcl-2 (B-cell Leukemia/Lymphoma 2) ELISA Kit
The Human BCL-2 (B-cell Leukemia/Lymphoma 2) ELISA Kit is specially designed for the precise measurement of BCL-2 levels in human serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring dependable and consistent results, and is well-suited for various research applications.BCL-2 is a key regulator of apoptosis, playing a crucial role in cell survival and resistance to programmed cell death.
Dysregulation of BCL-2 has been implicated in various diseases, including leukemia, lymphoma, and other cancers, making it a valuable biomarker for studying these conditions and potentially developing new treatments.Order the Human BCL-2 ELISA Kit today to advance your research in cancer biology, immunology, and molecular medicine.
Product Name:
Human Bcl-2 (B-cell Leukemia/Lymphoma 2) ELISA Kit
SKU:
HUES01400
Target:
Human Bcl-2 (B-cell Leukemia/Lymphoma 2)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.10 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human Bcl-2. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human Bcl-2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human Bcl-2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human Bcl-2. You can calculate the concentration of Human Bcl-2 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
89-102
90-102
97-114
Average (%)
95
97
104
1:4
Range (%)
100-113
87-103
94-105
Average (%)
105
94
100
1:8
Range (%)
95-109
85-100
95-110
Average (%)
102
91
101
1:16
Range (%)
96-111
82-94
94-107
Average (%)
103
89
100
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-101
96
EDTA plasma (n=5)
86-96
91
Cell culture media (n=5)
92-105
100
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.5
1.6
4.2
0.5
1.6
3.8
Standard deviation
0.03
0.08
0.15
0.03
0.08
0.2
C V (%)
6
5.0
3.57
6.0
5.0
5.26
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human Bcl-2 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human Bcl-2 in samples. No significant cross-reactivity or interference between Human Bcl-2 and analogues was observed.
