Human BCAT1 Recombinant Protein (RPPB1437)

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SKU:
RPPB1437
Product type:
Recombinant Protein
Size:
20ug
Species:
Human
Target:
BCAT1
Synonyms:
Branched chain amino-acid transaminase 1 cytosolic
Synonyms:
BCT1
Synonyms:
BCATC
Synonyms:
Protein ECA39
Source:
Escherichia Coli
Uniprot:
P54687
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Description

Product Name:Human BCAT1 Recombinant Protein
Product Code:RPPB1437
Size:20µg
Species:Human
Target:BCAT1
Synonyms:Branched chain amino-acid transaminase 1 cytosolic, BCT1, BCATC, Protein ECA39, placental protein 18, PP18, PNAS121, MECA39, EC 2.6.1.42.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered colorless solution.
Formulation:The BCAT1 solution (1mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 100mM Nacl, 1mM DTT and 20% glycerol.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 90% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MGSMKDCSNG CSAECTGEGG SKEVVGTFKA KDLIVTPATI LKEKPDPNNL VFGTVFTDHM LTVEWSSEFG WEKPHIKPLQ NLSLHPGSSA LHYAVELFEG LKAFRGVDNK IRLFQPNLNM DRMYRSAVRA TLPVFDKEEL LECIQQLVKL DQEWVPYSTS ASLYIRPTFI GTEPSLGVKK PTKALLFVLL SPVGPYFSSG TFNPVSLWAN PKYVRAWKGG TGDCKMGGNY GSSLFAQCEA VDNGCQQVLW LYGEDHQITE VGTMNLFLYW INEDGEEELA TPPLDGIILP GVTRRCILDL AHQWGEFKVS ERYLTMDDLT TALEGNRVRE MFGSGTACVV CPVSDILYKG ETIHIPTMEN GPKLASRILS KLTDIQYGRE ESDWTIVLS

BCAT1 catalyzes the leading reaction in the catabolism of the indispensable branched chain amino acids isoleucine, leucine and valine.

BCAT1 Recombinant produced in E. coli is a single polypeptide chain containing 409 amino acids (1-386) and having a molecular mass of 45.4kDa.BCAT1 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

UniProt Protein Function:BCAT1: Catalyzes the first reaction in the catabolism of the essential branched chain amino acids leucine, isoleucine, and valine. Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family. 4 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:EC 2.6.1.42; Amino Acid Metabolism - valine, leucine and isoleucine biosynthesis; Cofactor and Vitamin Metabolism - pantothenate and CoA biosynthesis; Amino Acid Metabolism - valine, leucine and isoleucine degradation; Transferase

Chromosomal Location of Human Ortholog: 12p12.1

Cellular Component: mitochondrion; cytosol

Molecular Function:identical protein binding; branched-chain-amino-acid transaminase activity

Biological Process: cell proliferation; branched chain family amino acid catabolic process; branched chain family amino acid biosynthetic process; G1/S transition of mitotic cell cycle

NCBI Summary:This gene encodes the cytosolic form of the enzyme branched-chain amino acid transaminase. This enzyme catalyzes the reversible transamination of branched-chain alpha-keto acids to branched-chain L-amino acids essential for cell growth. Two different clinical disorders have been attributed to a defect of branched-chain amino acid transamination: hypervalinemia and hyperleucine-isoleucinemia. As there is also a gene encoding a mitochondrial form of this enzyme, mutations in either gene may contribute to these disorders. Alternatively spliced transcript variants have been described. [provided by RefSeq, May 2010]
UniProt Code:P54687
NCBI GenInfo Identifier:215274162
NCBI Gene ID:586
NCBI Accession:P54687.3
UniProt Secondary Accession:P54687,Q68DQ7, Q96MY9, B3KY27, B7Z2M5, B7Z5L0, F5H5E4
UniProt Related Accession:P54687
Molecular Weight:386
NCBI Full Name:Branched-chain-amino-acid aminotransferase, cytosolic
NCBI Synonym Full Names:branched chain amino-acid transaminase 1, cytosolic
NCBI Official Symbol:BCAT1  
NCBI Official Synonym Symbols:BCT1; PP18; BCATC; ECA39; MECA39; PNAS121  
NCBI Protein Information:branched-chain-amino-acid aminotransferase, cytosolic; placental protein 18; branched chain aminotransferase 1, cytosolic
UniProt Protein Name:Branched-chain-amino-acid aminotransferase, cytosolic
UniProt Synonym Protein Names:Protein ECA39
Protein Family:Branched-chain-amino-acid aminotransferase
UniProt Gene Name:BCAT1  
UniProt Entry Name:BCAT1_HUMAN
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