The Human BCAN (Brevican) ELISA Kit is a cutting-edge solution for quantifying brevican levels in human biological samples such as serum, plasma, and cell culture supernatants. With its outstanding accuracy and precision, this kit delivers dependable and consistent results, making it perfect for various research purposes.Brevican is a key component involved in regulating the structure of the extracellular matrix in the central nervous system and plays a crucial role in synaptic plasticity.
Abnormal brevican expression has been associated with neurological disorders, making it a valuable biomarker for studying these conditions and exploring potential treatment strategies.With the Human BCAN ELISA Kit, researchers can explore the intricate mechanisms of brevican function and its implications in various diseases, paving the way for new insights and therapeutic discoveries.
Product Name:
Human BCAN (Brevican) ELISA Kit
SKU:
HUES01772
Target:
Human BCAN (Brevican)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human BCAN. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human BCAN and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human BCAN, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human BCAN. You can calculate the concentration of Human BCAN in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
92-106
98-113
90-103
Average (%)
98
105
98
1:4
Range (%)
89-103
81-95
88-101
Average (%)
94
86
94
1:8
Range (%)
87-101
81-93
82-93
Average (%)
94
87
88
1:16
Range (%)
92-106
86-96
89-101
Average (%)
99
91
95
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
86-100
91
EDTA plasma (n=5)
90-102
96
Cell culture media (n=5)
86-100
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
1.09
2.61
9.07
1.08
2.46
9.52
Standard deviation
0.06
0.15
0.28
0.07
0.12
0.4
C V (%)
5.5
5.75
3.09
6.48
4.88
4.2
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human BCAN concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human BCAN in samples. No significant cross-reactivity or interference between Human BCAN and analogues was observed.
