Human Basic Helix Loop Helix Domain Containing Protein B8 (BHLHB8) ELISA Kit
The Human Basic Helix-Loop-Helix Domain-Containing Protein B8 (BHLHB8) ELISA Kit is a highly sensitive and specific assay designed for the accurate measurement of BHLHB8 levels in human samples including serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it ideal for a variety of research applications.BHLHB8 is a transcription factor that plays a critical role in the regulation of gene expression and cell differentiation. It is involved in various cellular processes including neurogenesis, myogenesis, and circadian rhythm regulation.
Dysregulation of BHLHB8 has been implicated in neurodevelopmental disorders, cancer, and metabolic diseases, highlighting its importance as a potential biomarker for studying these conditions and developing new treatments.Overall, the Human Basic Helix-Loop-Helix Domain-Containing Protein B8 (BHLHB8) ELISA Kit is a valuable tool for researchers investigating the role of BHLHB8 in health and disease, offering precise and accurate measurements for comprehensive analysis.
Product Name:
Human Basic Helix Loop Helix Domain Containing Protein B8 (BHLHB8) ELISA Kit
Product Code:
HUDL00326
Size:
96 Assays
Target:
BHLHB8
Synonyms:
MIST1, bHLHa15, BHLHB15, Class A Basic Helix Loop Helix protein 15, Muscle, intestine and stomach expression 1
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
