The Human BAK1 (BCL2 Antagonist/Killer 1) CLIA Kit is a highly sensitive and specific assay designed for the quantitative detection of BAK1 levels in human serum, plasma, and cell culture supernatants. BAK1 is a key protein involved in apoptosis, regulating cell death and survival pathways.This kit provides accurate and reproducible results, making it a valuable tool for researchers studying cell death mechanisms, cancer, and other diseases where apoptosis plays a critical role.
With its high sensitivity and specificity, the Human BAK1 CLIA Kit is an essential resource for investigating the role of BAK1 in various biological processes and developing targeted therapies. Order your Human BAK1 CLIA Kit now and enhance your research efforts in understanding apoptosis and its implications in disease progression.
Product Name:
Human BAK1 (Bcl2 Antagonist/Killer 1) CLIA Kit
SKU:
HUES00383
Target:
Human BAK1 (Bcl2 Antagonist/Killer 1)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
0.09 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human BAK1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human BAK1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human BAK1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human BAK1. You can calculate the concentration of Human BAK1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
102-116
87-103
103-115
Average (%)
109
94
109
1:4
Range (%)
99-117
86-101
86-101
Average (%)
107
93
93
1:8
Range (%)
104-116
99-113
86-101
Average (%)
110
106
93
1:16
Range (%)
89-101
98-110
95-109
Average (%)
96
104
101
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
101-115
107
EDTA plasma (n=5)
85-96
90
Cell culture media (n=5)
92-108
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.47
0.84
4.13
0.5
0.83
3.95
Standard deviation
0.06
0.08
0.44
0.06
0.08
0.32
C V (%)
12.77
9.52
10.65
12.0
9.64
8.1
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human BAK1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human BAK1 in samples. No significant cross-reactivity or interference between Human BAK1 and analogues was observed.
