Human BAG3 (Bcl2 Associated Athanogene 3) ELISA Kit
The Human BAG3 (Bcl-2-associated athanogene 3) ELISA Kit is specifically designed for the accurate and sensitive detection of BAG3 levels in human samples such as serum, plasma, and cell culture supernatants. This kit provides researchers with a reliable and efficient tool for studying the role of BAG3 in various biological processes and diseases. BAG3 is a co-chaperone protein that plays a crucial role in regulating protein quality control, cell survival, and apoptosis. Dysregulation of BAG3 has been associated with various diseases, including cancer, neurodegenerative disorders, and cardiac diseases.
Therefore, studying BAG3 levels can provide valuable insights into the pathogenesis of these conditions and aid in the development of potential therapeutic strategies. With its high sensitivity and specificity, the Human BAG3 ELISA Kit from Assay Genie offers researchers a powerful tool for analyzing BAG3 levels in human samples, enabling precise and reproducible results for their research studies.
Product Name:
Human BAG3 (Bcl2 Associated Athanogene 3) ELISA Kit
SKU:
HUES01631
Target:
Human BAG3 (Bcl2 Associated Athanogene 3)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.10 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human BAG3. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human BAG3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human BAG3, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human BAG3. You can calculate the concentration of Human BAG3 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
92-105
91-102
94-107
Average (%)
99
96
102
1:4
Range (%)
91-106
81-93
87-99
Average (%)
97
87
92
1:8
Range (%)
85-101
81-92
87-99
Average (%)
92
87
93
1:16
Range (%)
93-106
82-92
80-95
Average (%)
100
87
87
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
85-95
90
EDTA plasma (n=5)
88-104
96
Cell culture media (n=5)
90-104
97
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.49
1.19
4.73
0.47
1.1
5.17
Standard deviation
0.03
0.07
0.21
0.03
0.07
0.18
C V (%)
6.12
5.88
4.44
6.38
6.36
3.48
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human BAG3 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human BAG3 in samples. No significant cross-reactivity or interference between Human BAG3 and analogues was observed.
