Human BAFFR (B Cell Activating Factor Receptor) ELISA Kit
The Human BAFFR (B-cell Activating Factor Receptor) ELISA Kit is a powerful tool for measuring levels of BAFFR in human samples, including serum, plasma, and cell culture supernatants. With exceptional sensitivity and specificity, this kit provides accurate and reproducible results for various research applications.BAFFR is a key receptor involved in regulating B-cell activation and survival, making it an important target for studying immune responses, autoimmune diseases, and potential therapeutic interventions.
By accurately quantifying BAFFR levels, researchers can gain valuable insights into the role of this receptor in health and disease.Whether investigating immunological pathways, exploring disease mechanisms, or developing new treatments, the Human BAFFR ELISA Kit offers a reliable and efficient solution for detecting and measuring BAFFR levels in human samples. Upgrade your research with this cutting-edge kit and unlock new possibilities in the field of immunology.
Product Name:
Human BAFFR (B Cell Activating Factor Receptor) ELISA Kit
SKU:
HUES01735
Target:
Human BAFFR (B Cell Activating Factor Receptor)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.10 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human BAFFR. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human BAFFR and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human BAFFR, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human BAFFR. You can calculate the concentration of Human BAFFR in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
87-97
85-96
86-99
Average (%)
92
91
93
1:4
Range (%)
90-104
84-95
82-95
Average (%)
96
90
89
1:8
Range (%)
93-105
87-100
86-101
Average (%)
99
92
92
1:16
Range (%)
87-100
86-100
82-94
Average (%)
92
92
87
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-104
98
EDTA plasma (n=5)
90-104
96
Cell culture media (n=5)
95-107
100
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.47
1.43
3.45
0.44
1.38
3.24
Standard deviation
0.03
0.08
0.12
0.03
0.08
0.12
C V (%)
6.38
5.59
3.48
6.82
5.8
3.7
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human BAFFR concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human BAFFR in samples. No significant cross-reactivity or interference between Human BAFFR and analogues was observed.
