Human BAFF Recombinant Protein (RPPB0086)

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SKU:
RPPB0086
Product type:
Recombinant Protein
Size:
5ug
Species:
Human
Target:
BAFF
Synonyms:
BAFF
Synonyms:
BLYS
Synonyms:
CD257
Synonyms:
TALL1
Source:
Nicotiana benthamiana plant
Uniprot:
Q9Y275
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Description

Product Name:Human BAFF Recombinant Protein
Product Code:RPPB0086
Size:5µg
Species:Human
Target:BAFF
Synonyms:BAFF, BLYS, CD257, TALL1, THANK, ZTNF4, TALL-1, TNFSF20, TNFSF13B, B-cell Activating Factor.
Source:Nicotiana benthamiana plant
Physical Appearance:Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation:Lyophilized from 1mg/ml solution in 20 mM PBS buffer pH 7 and 0.2 M NaCl.
Solubility:It is recommended to reconstitute the lyophilized BAFF in sterile 18M-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Stability:Lyophilized BAFF although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution BAFF should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Purity:Greater than 97.0% as determined by Analysis by SDS-PAGE.
Amino Acid Sequence:HHHHHHHHHH AVQGPEETVT QDCLQLIADS ETPTIQKGSY TFVPWLLSFK RGSALEEKEN KILVKETGYF FIYGQVLYTD KTYAMGHLIQ RKKVHVFGDE LSLVTLFRCI QNMPETLPNN SCYSAGIAKL EEGDELQLAI PRENAQISLD GDVTFFGALK LL
Biological Activity:The activity is determined by dose-dependent stimulation of proliferation B cell from Human PBMC. Cell proliferation was measured by MTT method. *activity results may vary with PBMC donors. ED50 ? 50ng/ml

BAFF binds to tnfrsf13b/taci and tnfrsf17/bcma. Tnfsf13/april binds to the same 2 receptors, together, they form a 2 ligands -2 receptors pathway involved in the stimulation of b- and t-cell function and the regulation of humoral immunity.A third b-cell specific baff-receptor (baffr/br3) promotes the survival of mature b-cells and the b-cell response.B Lymphocyte Stimulator functions as a potent B-cell growth factor in costimulation assays. Administration of BAFF Human recombinant to mice disrupts splenic B-cell and T-cell zones and results in elevated levels of serum immunoglobulin.

BAFF human Recombinant produced in Nicotiana benthamiana plant is a single glycosilated polypeptide chain containing 151 amino acids fragment (134-285).BAFF (C830H1277N223O242S5) is fused to a 10-His-tag at the N-terminal having the total molecular mass of 18-20kDa and purified by standard chromatographic techniques.

UniProt Protein Function:BAFF: Cytokine that binds to TNFRSF13B/TACI and TNFRSF17/BCMA. TNFSF13/APRIL binds to the same 2 receptors. Together, they form a 2 ligands -2 receptors pathway involved in the stimulation of B- and T-cell function and the regulation of humoral immunity. A third B-cell specific BAFF-receptor (BAFFR/BR3) promotes the survival of mature B-cells and the B-cell response. Belongs to the tumor necrosis factor family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Apoptosis; Membrane protein, integral; Cytokine; Cell cycle regulation

Chromosomal Location of Human Ortholog: 13q32-q34

Cellular Component: extracellular space; perinuclear region of cytoplasm; cytoplasm; plasma membrane; integral to membrane

Molecular Function:protein binding; cytokine activity; tumor necrosis factor receptor binding; receptor binding

Biological Process: cell proliferation; B cell costimulation; B cell homeostasis; immunoglobulin secretion; T cell costimulation; positive regulation of cell proliferation; positive regulation of germinal center formation; positive regulation of B cell proliferation; immune response; positive regulation of T cell proliferation; signal transduction

NCBI Summary:The protein encoded by this gene is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This cytokine is a ligand for receptors TNFRSF13B/TACI, TNFRSF17/BCMA, and TNFRSF13C/BAFFR. This cytokine is expressed in B cell lineage cells, and acts as a potent B cell activator. It has been also shown to play an important role in the proliferation and differentiation of B cells. Alternatively spliced transcript variants encoding distinct isoforms have been identified. [provided by RefSeq, Mar 2011]
UniProt Code:Q9Y275
NCBI GenInfo Identifier:13124573
NCBI Gene ID:10673
NCBI Accession:Q9Y275.1
UniProt Secondary Accession:Q9Y275,Q6FHD6, Q7Z5J2, E0ADT7,
UniProt Related Accession:Q9Y275
Molecular Weight:17,578 Da
NCBI Full Name:Tumor necrosis factor ligand superfamily member 13B
NCBI Synonym Full Names:tumor necrosis factor (ligand) superfamily, member 13b
NCBI Official Symbol:TNFSF13B  
NCBI Official Synonym Symbols:DTL; BAFF; BLYS; CD257; TALL1; THANK; ZTNF4; TALL-1; TNFSF20  
NCBI Protein Information:tumor necrosis factor ligand superfamily member 13B; delta BAFF; Delta4 BAFF; B-lymphocyte stimulator; B-cell-activating factor; ApoL related ligand TALL-1; TNF homolog that activates apoptosis; dendritic cell-derived TNF-like molecule; tumor necrosis factor-like protein ZTNF4; TNF and ApoL-related leukocyte expressed ligand 1; tumor necrosis factor (ligand) superfamily, member 20
UniProt Protein Name:Tumor necrosis factor ligand superfamily member 13B
UniProt Synonym Protein Names:B lymphocyte stimulator; BLyS; B-cell-activating factor; BAFF; Dendritic cell-derived TNF-like molecule; TNF- and APOL-related leukocyte expressed ligand 1; TALL-1; CD_antigen: CD257Cleaved into the following 2 chains:Tumor necrosis factor ligand superfamily member 13b, membrane form; Tumor necrosis factor ligand superfamily member 13b, soluble form
UniProt Gene Name:TNFSF13B  
UniProt Entry Name:TN13B_HUMAN
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