Human BAFF/CD257 (B-Cell Activating Factor) ELISA Kit
The Human BAFF (B Cell Activating Factor) ELISA Kit is a powerful tool for researchers looking to accurately measure BAFF levels in human samples such as serum, plasma, and cell culture supernatants. With exceptional sensitivity and specificity, this kit delivers reliable and reproducible results for a variety of research applications.BAFF, also known as CD257, is a key cytokine that plays a critical role in B cell survival, proliferation, and maturation. Dysregulation of BAFF has been linked to autoimmune diseases such as lupus and rheumatoid arthritis, making it a valuable biomarker for studying these conditions and developing targeted therapies.
By utilizing the Human BAFF ELISA Kit, researchers can gain valuable insights into the role of BAFF in immune system function and disease pathology, ultimately advancing our understanding of autoimmune disorders and facilitating the development of innovative treatments.
Product Name:
Human BAFF/CD257 (B-Cell Activating Factor) ELISA Kit
SKU:
HUES01296
Target:
Human BAFF/CD257 (B-Cell Activating Factor)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human BAFF/CD257. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human BAFF/CD257 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human BAFF/CD257, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human BAFF/CD257. You can calculate the concentration of Human BAFF/CD257 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
92-105
95-108
91-103
Average (%)
100
101
97
1:4
Range (%)
91-108
86-99
87-102
Average (%)
99
93
94
1:8
Range (%)
86-99
85-97
85-100
Average (%)
93
90
91
1:16
Range (%)
91-106
86-100
85-96
Average (%)
98
92
90
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
87-101
94
EDTA plasma (n=5)
86-100
91
Cell culture media (n=5)
90-105
96
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
185.79
600.16
1343.28
173.82
543.69
1425.24
Standard deviation
11.65
35.47
54.13
11.94
28.71
45.04
C V (%)
6.27
5.91
4.03
6.87
5.28
3.16
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human BAFF/CD257 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human BAFF/CD257 in samples. No significant cross-reactivity or interference between Human BAFF/CD257 and analogues was observed.
