Beta-site APP-cleaving enzyme 1 (BACE1) is an aspartic-acid protease important in the formation of myelin sheaths in peripheral nerve cells. In the brain; This protein is expressed highly in the substantia nigra; locus coruleus and medulla oblongata. Strong BACE1 expression has also been described in pancreatic tissue. BACE1 has a pivotal role in the pathogenesis of Alzheimer's disease. In Alzheimer's disease patients; BACE1 levels were elevated although mRNA levels were not changed. It has been found that BACE1 gene expression is controlled by a TATA-less promoter. The translational repression as a new mechanism controlling its expression. And the low concentrations of Ca(2+) (microM range) significantly increased the proteolytic activity of BACE1. Furthermore; BACE1 protein is ubiquitinated; and the degradation of BACE1 proteins and amyloid precursor protein processing are regulated by the ubiquitin-proteasome pathway. It has also been identified as the rate limiting enzyme for amyloid-beta-peptide (Abeta) production.
Product Name:
Human BACE1/ASP2 Recombinant Protein (RPES0508)
Product Code:
RPES0508
Size:
50µg
Species:
Human
Expressed Host:
HEK293 Cells
Synonyms:
ASP2,BACE,HSPC104
Accession:
NP_036236.1
Sequence:
Met 1-Thr 457
Fusion tag:
Activity:
Measured by its ability to cleave a fluorescent peptide substrate Mca-Ser-Glu-Val-Asn-Leu-Asp-Ala-Glu-Phe-Arg-Lys(Dpn)-Arg-Arg-NH2 (Catalog# ES004; R&D Systems). Cleavage of ES004 can be measured using excitation and emission wavelength of 320 and 405 nm; respectively. The specific activity is >3.5 pmoles/min/µg.
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
The mature form of human BACE1 (NP_036236.1) extracellular domain (Met1-Thr 457) with a quinary-aa peptide (DDDDK) at the C-terminus was expressed and purified.
Purity:
> 95 % as determined by reducing SDS-PAGE.
Mol Mass:
49 kDa
AP Mol Mass:
65 kDa
Formulation:
Lyophilized from sterile 50mM Tris; 100mM NaCl; pH 8.0
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
