Programmed death ligand 2 (PD-L2); also referred to as B7-DC and CD273; is a member of the B7 family of proteins including B7-1; B7-2; B7-H2; B7-H1 (PD-L1); and B7-H3. PD-L2 is a type I membrane protein and structurally consists of an extracellular region containing one V-like and one C-like Ig domain; a transmembrane region; and a short cytoplasmic domain. PD-L2 is expressed on antigen presenting cells; placental endothelium and medullary thymic epithelial cells; and can be induced by LPS in B cells; INF-γ in monocytes; or LPS plus IFN-γ in dendritic cells. The CD28 and B7 protein families are critical regulators of immune responses. PD-L2 and PD-L1 are two ligands for PD-1; member of the CD28/CTLA4 family expressed on activated lymphoid cells; and thus provide signals for regulating T cell activation and immune tolerance. The interaction of B7-DC/PD-1 exhibited a 2-6-fold higher affinity compared with the interaction of B7-H1/PD-1.Immune CheckpointImmune Checkpoint Detection: Antibodies Immune Checkpoint Detection: ELISA Antibodies Immune Checkpoint Detection: FCM Antibodies Immune Checkpoint Detection: WB AntibodiesImmune Checkpoint ProteinsImmune Checkpoint Targets Co-inhibitory Immune Checkpoint Targets Immunotherapy Cancer Immunotherapy Targeted Therapy
Product Name:
Human B7-DC/PD-L2/CD273 Recombinant Protein (RPES4709)
Measured by its binding ability in a functional ELISA. Immobilized human PD1 at 2 µg/ml (100 ?l/well) can bind human PD-L2 with a linear ranger of 0.0064-0.16 µg/ml.
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
A DNA sequence encoding the extracellular domain (Met 1-Pro 219) of human PD-L2 (NP_079515.2) was fused with the C-terminal His-tagged Fc region of human IgG1 at the C-terminus.
Purity:
> 97 % as determined by reducing SDS-PAGE.
Mol Mass:
50.4 kDa
AP Mol Mass:
70-80 kDa
Formulation:
Lyophilized from sterile PBS; pH 7.4
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
