Human B-Raf Proto Oncogene Serine/Threonine Protein Kinase (BRAF) ELISA Kit
The Human B-RAF Proto-Oncogene Serine/Threonine Protein Kinase (BRAF) ELISA Kit is a cutting-edge tool designed for the precise quantification of BRAF levels in human samples including serum, plasma, and cell culture supernatants. This advanced kit offers unparalleled sensitivity and specificity, ensuring accurate and consistent results for a variety of research applications.BRAF is a critical enzyme involved in cell signaling and regulation, playing a key role in various pathways related to cell growth and proliferation.
Dysregulation of BRAF has been linked to a number of diseases, including certain types of cancer, making it a valuable biomarker for studying disease progression and potential therapeutic interventions.With its superior performance and reliability, the BRAF ELISA Kit from Assay Genie is a must-have tool for researchers investigating the role of BRAF in health and disease. Trust in Assay Genie to provide you with the tools you need for groundbreaking research in the field of oncology and beyond.
Product Name:
Human B-Raf Proto Oncogene Serine/Threonine Protein Kinase (BRAF) ELISA Kit
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
