BCL11A-L,BCL11A-S,BCL11A-XL,CTIP1,EVI9,Ecotropic Viral Integration Site 9,COUP-TF-interacting protein 1,Ecotropic viral integration site 9 homolog,Zinc finger protein 856
Reactivity:
Human
Sample Type:
Tissue Homogenates, Cell Lysates And Other Biological Fluids
The Human BCL11A (B-Cell CLL/Lymphoma 11A) ELISA Kit is specially designed for the precise measurement of BCL11A levels in human serum, plasma, and cell culture supernatants. This ELISA kit boasts exceptional sensitivity and specificity, guaranteeing dependable and consistent results, making it well-suited for various research applications.BCL11A is a critical protein involved in B-cell development and function, playing a key role in B-cell lymphoma and other hematologic malignancies.
Understanding BCL11A levels can provide valuable insights into the pathogenesis of these diseases and aid in the development of novel therapeutic strategies. This ELISA kit provides researchers with a valuable tool to accurately quantify BCL11A levels, enabling in-depth investigations into its role in health and disease. With its user-friendly protocol and robust performance, the Human BCL11A ELISA Kit is an essential resource for advancing research in oncology and hematology.
Product Name:
Human B-Cell CLL/Lymphoma 11A (Bcl11A) ELISA Kit
Product Code:
HUDL00317
Size:
96 Assays
Target:
Bcl11A
Synonyms:
BCL11A-L, BCL11A-S, BCL11A-XL, CTIP1, EVI9, Ecotropic Viral Integration Site 9, COUP-TF-interacting protein 1, Ecotropic viral integration site 9 homolog, Zinc finger protein 856
Detection Method:
Sandwich
Range:
0.312-20ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
83-100
91
EDTA plasma (n=5)
90-103
96
Heparin plasma (n=5)
89-102
95
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
