The Human AZGP1 (Zinc Alpha-2-Glycoprotein) ELISA Kit is specifically designed for the accurate measurement of AZGP1 levels in human serum, plasma, and cell culture supernatants. This kit is highly sensitive and specific, providing reliable and reproducible results for a variety of research applications.AZGP1 is an important protein involved in various physiological processes, including lipid metabolism, cell migration, and tumor suppression.
Abnormal AZGP1 levels have been linked to conditions such as obesity, cancer, and metabolic disorders, making it a valuable biomarker for studying these conditions and developing targeted therapies.With its high-quality components and user-friendly protocol, the Human AZGP1 ELISA Kit is an essential tool for researchers studying AZGP1 function and its role in human health and disease.
Product Name:
Human AZGP1 (Zinc-alpha-2-glycoprotein) ELISA Kit
SKU:
HUES02556
Target:
Human AZGP1 (Zinc-alpha-2-glycoprotein)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.94 ng/mL
Detection range:
1.56-100 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human AZGP1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human AZGP1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human AZGP1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human AZGP1. You can calculate the concentration of Human AZGP1 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
92-106
90-103
91-104
Average (%)
98
97
97
1:4
Range (%)
89-100
80-94
85-100
Average (%)
95
86
92
1:8
Range (%)
85-98
84-94
81-93
Average (%)
92
88
87
1:16
Range (%)
90-104
83-97
82-93
Average (%)
95
89
87
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-106
98
EDTA plasma (n=5)
90-104
96
Cell culture media (n=5)
94-110
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
5.31
12.52
42.61
5.56
13.46
41.1
Standard deviation
0.34
0.56
1.91
0.38
0.72
1.85
C V (%)
6.4
4.47
4.48
6.83
5.35
4.5
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human AZGP1 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human AZGP1 in samples. No significant cross-reactivity or interference between Human AZGP1 and analogues was observed.
