The Human ATXN2 (Ataxin-2) ELISA Kit is specifically designed for the quantitative detection of ATXN2 levels in human biological samples such as serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring accurate and reliable results for a variety of research applications.Ataxin-2 is a protein that plays a crucial role in the regulation of RNA metabolism and protein synthesis. Dysregulation of ATXN2 has been associated with neurodegenerative diseases such as spinocerebellar ataxia type 2 (SCA2) and amyotrophic lateral sclerosis (ALS), making it a valuable biomarker for studying these conditions and potentially developing targeted therapies.
By using the Human ATXN2 ELISA Kit, researchers can effectively measure ATXN2 levels in human samples, providing valuable insights into the pathogenesis of neurodegenerative disorders and facilitating the development of new treatment strategies.
Product Name:
Human ATXN2 (Ataxin 2) ELISA Kit
SKU:
HUES01716
Target:
Human ATXN2 (Ataxin 2)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.47 ng/mL
Detection range:
0.78-50 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human ATXN2. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ATXN2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ATXN2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human ATXN2. You can calculate the concentration of Human ATXN2 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
94-109
93-106
88-104
Average (%)
101
100
95
1:4
Range (%)
93-108
87-102
86-97
Average (%)
98
93
91
1:8
Range (%)
91-102
86-100
81-95
Average (%)
97
92
87
1:16
Range (%)
90-105
84-96
83-95
Average (%)
96
89
90
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-107
100
EDTA plasma (n=5)
91-107
98
Cell culture media (n=5)
91-104
97
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
2.67
4.9
22.65
2.75
5.1
21.61
Standard deviation
0.15
0.25
0.83
0.15
0.29
0.67
C V (%)
5.62
5.1
3.66
5.45
5.69
3.1
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human ATXN2 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human ATXN2 in samples. No significant cross-reactivity or interference between Human ATXN2 and analogues was observed.
