The Human ATXN1 (Ataxin-1) ELISA Kit is specifically designed for the precise measurement of ATXN1 levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and reproducible results for various research applications.ATXN1 is a key protein involved in neurodegenerative disorders such as spinocerebellar ataxia type 1 (SCA1).
Dysregulation of ATXN1 has been linked to the pathogenesis of SCA1, making it a valuable biomarker for studying and understanding these disorders. The Human ATXN1 ELISA Kit provides researchers with a reliable tool for investigating the role of ATXN1 in neurodegenerative diseases and exploring potential therapeutic strategies.
Product Name:
Human ATXN1 (Ataxin 1) ELISA Kit
SKU:
HUES01715
Target:
Human ATXN1 (Ataxin 1)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human ATXN1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ATXN1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ATXN1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human ATXN1. You can calculate the concentration of Human ATXN1 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
86-101
98-109
86-99
Average (%)
92
104
93
1:4
Range (%)
92-104
85-97
89-101
Average (%)
98
92
94
1:8
Range (%)
92-105
85-98
81-96
Average (%)
97
91
88
1:16
Range (%)
88-100
79-94
84-99
Average (%)
95
86
90
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-104
97
EDTA plasma (n=5)
85-97
92
Cell culture media (n=5)
87-100
94
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
1.09
3.08
6.98
1.11
2.92
6.58
Standard deviation
0.06
0.18
0.21
0.06
0.12
0.31
C V (%)
5.5
5.84
3.01
5.41
4.11
4.71
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human ATXN1 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human ATXN1 in samples. No significant cross-reactivity or interference between Human ATXN1 and analogues was observed.
