Human ATPase, Na+/K+ Transporting Beta 4 Polypeptide (ATP1b4) ELISA Kit
The Human ATPase Na+/K+ Transporting Beta 4 Polypeptide (ATP1B4) ELISA Kit is specifically designed for the accurate quantitative measurement of ATP1B4 levels in human serum, plasma, and cell culture supernatants. This kit boasts high sensitivity and specificity, ensuring precise and reliable results for your research needs.ATP1B4 is a key component of the Na+/K+ ATPase enzyme complex, which plays a crucial role in maintaining cellular ion balance and regulating cell volume. Dysregulation of ATP1B4 has been implicated in various diseases, including hypertension, heart failure, and neurological disorders, making it a valuable biomarker for studying these conditions and potentially developing targeted therapies.
With its easy-to-use protocol and exceptional performance, the Human ATP1B4 ELISA Kit is an essential tool for researchers investigating the function and significance of ATP1B4 in health and disease. Order yours today and take your research to the next level.
Product Name:
Human ATPase, Na+/K+ Transporting Beta 4 Polypeptide (ATP1b4) ELISA Kit
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
83-99
91
EDTA plasma (n=5)
80-99
89
Heparin plasma (n=5)
82-99
90
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
