Human ATPase, Ca++ Transporting, Cardiac Muscle, Slow Twitch 2 (ATP2A2) ELISA Kit

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SKU:
HUDL00285
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P16615
Sensitivity:
0.126ng/mL
Range:
0.312-20ng/mL
ELISA Type:
Sandwich
Synonyms:
ATP2B,DAR,SERCA2,Calcium pump 2,Endoplasmic reticulum class 1/2 Ca(2+) ATPase,Calcium-transporting ATPase sarcoplasmic reticulum type, slow twitch skeletal muscle
Reactivity:
Human
Sample Type:
Tissue Homogenates, Cell Lysates And Other Biological Fluids
Frequently bought together:

Description

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Human ATPase, Ca++ Transporting, Cardiac Muscle, Slow Twitch 2 (ATP2A2) ELISA Kit

The Human ATPase Ca++ transporting cardiac muscle slow twitch 2 (ATP2A2) ELISA Kit is a reliable and accurate tool for the detection of ATP2A2 levels in human serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reproducible results for a variety of research applications.ATP2A2 is a critical enzyme involved in calcium homeostasis in cardiac muscle cells, playing a key role in muscle contraction and relaxation. Dysregulation of ATP2A2 has been implicated in various cardiac disorders, making it a valuable biomarker for studying these conditions and developing potential therapies.

With its advanced technology and user-friendly design, the Human ATP2A2 ELISA Kit is an essential tool for researchers investigating the role of ATP2A2 in cardiac muscle function and its implications in cardiovascular diseases. Trust in this kit to deliver reliable and accurate data for your research needs.

Product Name:Human ATPase, Ca++ Transporting, Cardiac Muscle, Slow Twitch 2 (ATP2A2) ELISA Kit
Product Code:HUDL00285
Size:96 Assays
Target:ATP2A2
Synonyms:ATP2B, DAR, SERCA2, Calcium pump 2, Endoplasmic reticulum class 1/2 Ca(2+) ATPase, Calcium-transporting ATPase sarcoplasmic reticulum type, slow twitch skeletal muscle
Detection Method:Sandwich
Range:0.312-20ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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